Minute™ Total Protein Extraction Kit

(for Animal Cultured Cells and Tissues, US Patent # 9,580,462)

MinuteTM total protein extraction kit for animal cultured cells and tissues is the most advanced next generation protein extraction tool for super-fast protein extraction without protein loss and altered protein profile. More and more evidences have shown that the most commonly used RIPA buffer can cause unpredictable protein loss, resulting in questionable data interpretation. This problem is fully resolved by the patented spin column based technologies. Coupled with much stronger lysis buffers, proteins from tough tissues such as skin, blood vessel, muscle, etc. can also be extracted efficiently. Due to the use of the proprietary protein extraction filter cartridges, the extraction volume can be as low as 20 µl - a very useful feature in situations where starting material is a limiting factor. This kit provides both denaturing and native cell lysis buffers so users can select according to specific applications. Total proteins can be extracted from cultured cells/tissues in 1-8 min with high yield (2-8 mg/ml).

Selected references related to protein loss by RIPA buffer:
1. Bai,B., and Laiho, M. (2012) Proteomics. 12:3044-3048
2. Mukhopadhyay, C. et al. (2016) PNAS 5:8228-8237
3. Li, Q. (2016) Biotechniques. 61:327
4. Ngoka, L. CM. (2008) Proteome Science. 6:30

Cat# SD-001/SN-002   Price:$198 (50 preps)  

Invent Biotechnologies' Minute™ total protein extraction kit for animal cultured cells and tissues Test Results
Protein profiles of animal tissues extracted by denaturing lysis buffer and native lysis buffer. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, drosophila larvae; lane 2, drosophila pupa; 3, adult drosophila; 4, gold fish muscle and 5, mouse liver

Kit Components

Item Amount Part #
Denaturing Cell Lysis Buffer 25 ml SD-001-L
Native cell lysis buffer 25 ml SN-002-L
Protein Extraction Filter Cartridges 50 P-001
Collection Tubes with Cap 50 P-002
plastic rods 4 P-003