Minute™ Single Cell Isolation Kit

(For fresh and fixed tissues)

Invent Biotechnologies Minute™ single cell isolation kit is composed of optimized tissue disaggregation buffers and specially designed filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate single cells from fresh animal tissues with high cell viability. It can also be used for isolating nuclei from tissues briefly fixed with formaldehyde for chromosome immunoprecipitation (ChIP). The tissue disaggregation buffers are formulated to gently disaggregate animal tissues. The buffers don’t contain any proteinases and EDTA that may have adverse effects on cell surface markers. Due to the use of filter cartridges with pre-defined pore size and specially formulated buffer system single cell suspension can be obtained from fresh tissues in less than 10 min. The efficacy of the kit is tissue type-dependent. This kit is especially effective for lymphoid tissues such as spleen, thymus, and lymph node. For fresh tissues with high amount of connective tissue content the efficacy is variable. This kit is not recommended for fresh tissues such as muscle, intestine and lungs. For fixed tissues, tissue types are less critical.

Cat# SC-012   Price:$198 (50 preps)  

Single Cell # Per 50 mg fresh mouse tissue and viability
Tissues Brain Kidney Liver Lung Spleen
Cell # in Millions 4-5 5-6 5-6 6-7 15-16
Viability (%) 40-50 50-55 70-75 70-80 90-95
  Single Cell# Per 50 mg formaldehyde fixed mouse tissue
Tissues Brain Kidney Liver Lung Spleen
Cell # in Millions 4-5 4-5 4-5 5-6 13-15

Kit Components

Item Amount Part #
Buffer A (for non-fixed tissues) 25 ml SC-012-N
Buffer B (for fixed tissues) 25 ml SC-012-F
Cell isolation filter cartridges 50 P-001
Collection Tubes with Cap 50 P-002
plastic rods 4 P-003