Minute™ Detergent-free nuclei isolation kit
Invent Biotechnologies MinuteTM detergent-free nuclei isolation kit is designed to rapidly isolate intact nuclei from animal cultured cells or tissues (fresh or frozen). Unlike many other methods in which detergents are used to lyse the cell membranes, intact nuclei can be isolated from the samples using this patented technology in less than 20 min without using any detergent and a tissue homogenizer. One major problem associates with the use of detergent is the tendency of nuclei aggregation, which is difficult to be separated into single nucleus. To overcome this shortcoming we have developed this detergent-free nuclei isolation kit. The sample is first incubated with buffer A that sensitizes the cells for mechanical disruption. The cell suspension is rapidly passing through a specially designed tilter cartridge. The cell membranes are ruptured when passing through the filter leaving intact native nuclei in the flow through. The nuclei are separated from other small cell debris by low speed centrifugation using a proprietary buffer B.
Price:$238 (20 preps)
|Buffer A||15 ml|
|Buffer B||30 ml|
|Protein extraction filter cartridges||20|