Minute™ Nuclei and Cytosol Isolation Kit for Adipose Tissues

$298.00 (20 preps)

Adipocytes are the major energy storage sites in the body and they also have critical endocrine
functions. Therefore, understanding the development and function of adipocytes are essential to
understanding metabolic homeostasis under physiological and pathological conditions.
Fractionation of cellular components into nucleus and cytosolic fraction is a common
practice in the lab. However, when it comes to adipocytes, separation of these two
fractions are much more difficult because of high concentration of lipid droplet and low
protein content of adipocytes. Methods reported in the literature are tedious and time
consuming and as much as 50 grams of tissue are required. This kit provides a very
rapid and simple method for obtaining high purity nuclei from adipose tissues and most
important of all, only milligram amounts of tissues are required making it possible to
isolate nuclei and cytosol from small animal and biopsy samples. The intact nuclei
isolated have many applications such as single nucleus RNA sequencing, SDS-PAGE,
immunoblotting, ELISA, IP, protein localization, gel mobility shift assays, 2-D gels and other applications.

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  • Catalog #: AN-029
  • Manufactured by: Invent Biotechnologies, Inc.

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