Minute™ Adipose Tissue Fractionation Kit

$298.00 (20 preps)

In average, total cellular proteins account for less than 2% of adipose tissues. Fractionation of adipose tissue is technically very challenging due to its high lipid and low protein contents. Adipose tissue especially white adipose tissue (WAT) has been recognized as an important endocrine and inflammation organ in addition to its energy storage function. Fractionation and analysis of proteins from adipose tissues are critical for understanding many physiological/pathological conditions. The water-oil emulsion present in biological sample is notoriously difficult to separate. We have developed a novel technology to deal with this issue. A porous filter with unique surface property and pre-defined pore size coupling with a specially formulated detergent-free fractionation buffer is the key to fractionate adipose tissue into two fractions: water-soluble protein fraction containing mainly cytosolic proteins and water insoluble fraction containing mainly plasma membrane, and organelles such as mitochondria. The buffers used in this kit are free of primary amine, detergent and reducing agents. Isolated proteins are compatible with all downstream applications including TMT labeling, enzyme digestion, MS analysis and other applications.

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  • Catalog #: AF-023

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