Minute™ Yeast Mitochondria Enrichment Kit

$238.00 (50 preps)

Traditional protocols for yeast mitochondria isolation/extraction involve a range of centrifugation-based subcellular fractionation procedures. Typically the techniques include spheroplast preparation, glass-bead lysis using a homogenization instrument, differential centrifugation and several density gradient procedures using a variety of gradient media with ultracentrifugation. The procedures are very tedious and time consuming. Here we feature a simple and rapid protocol for yeast mitochondria enrichment. The procedure is gentle and instrument-free. Nativ mitochondrial proteins can be isolated from yeast in about one hour without ultracentrifugation. This kit contains optimized detergent-free protein extraction buffers. The protein yield is in the range of 150-250 µg/sample. The materials provided are sufficient for 50 extractions.

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  • Catalog #: YM-017

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