Welcome!

Invent Biotechnologies features the Minute™ line of protein extraction kits. Our online catalog allows you to order kits easily and securely. Confirmed orders are shipped promptly and carefully to assure your complete satisfaction.




Minute™ Total Protein Extraction Kit for Animal Cultured Cells and Tissues

... more info
Minute™ Total Protein Extraction Kit for Animal Cultured Cells and Tissues
Catalog #: SD-001/SN-002
Price: $198 (50 preps)

Weight: 0.5lbs

Date Added: Friday 15 April, 2011
Invent Biotechnologies' Minute™ Total Protein Extraction Kit for Animal Cultured Cells and Tissues ... more info

Minute™ Cytoplasmic & Nuclear Extraction Kit

... more info
Minute™ Cytoplasmic & Nuclear Extraction Kit
Catalog #: SC-003
Price: $198 (50 preps)

Weight: 0lbs

Date Added: Saturday 16 April, 2011
Invent Biotechnologies Minute™ Cytoplasmic and Nuclear Extraction Kit is composed of optimized cytoplamic extraction buffer, nuclear protein ... more info

Minute™ Bacterial Total Protein Extraction Kit

... more info
Minute™ Bacterial Total Protein Extraction Kit
Catalog #: SB-004
Price: $198 (50 preps)

Weight: 0.5lbs

Date Added: Saturday 16 April, 2011
Invent Biotechnologies' Minute™ bacterial total protein extraction kit is composed of optimized denaturing cell lysis buffers and protein extraction ... more info

Minute™ Plasma Membrane Isolation Kit

... more info
Minute™ Plasma Membrane Isolation Kit
Catalog #: SM-005
Price: $298 (50 preps)

Weight: 0.5lbs

Date Added: Wednesday 22, 2012
Invent Biotechnologies Minute™ plasma membrane isolation kit is composed of optimized buffers (detergent and EDTA free) and protein extraction filter cartridges with 2.0 ml collection tubes. ... more info

Minute™ Detergent-Free Protein Extraction Kit For Animal Cultured Cells and Tissues

... more info
Minute™ Detergent-Free Protein Extraction Kit For Animal Cultured Cells and Tissues
Catalog #: SN-006
Price: $198 (50 preps)

Weight: 0.5lbs

Date Added: April 28, 2012
Invent Biotechnologies Minute™ detergent-free total protein extraction kit is composed of optimized cell lysis buffer and protein extraction filter cartridges with 2.0 ml collection tubes. ... more info

Minute™ Mitochondria Isolation Kit For Mammalian Cells And Tissues

... more info
Minute™ Mitochondria Isolation Kit For Mammalian Cells And Tissues
Catalog #: MP-007
Price: $298 (50 preps)

Weight: 0.5lbs

Date Added: April 28, 2012
Invent Biotechnologies Minute™ Mitochondria Isolation Kit For Mammalian Cells And Tissues is composed of optimized buffers and protein extraction filter cartridges with 2.0 ml collection tubes. ... more info

Minute™ Total Protein Extraction Kit for Plant Tissues

... more info
Minute™ Total Protein Extraction Kit for Plant Tissues
Catalog #: SD-008/SN-009
Price: $198 (50 preps)

Weight: 0.5lbs

Date Added: April 28, 2012
Invent Biotechnologies Minute™ total protein extraction kit for plant tissues is composed of optimized protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. ... more info

Minute™ Detergent-Free Plant Protein Extraction Kit

... more info
Minute™ Detergent-Free Plant Protein Extraction Kit
Catalog #: SN-010
Price: $198 (50 preps)

Weight: 0.5lbs

Date Added: April 28, 2012
Invent Biotechnologies Minute™ detergent-free plant protein extraction kit is composed of optimized protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. ... more info

Minute™ Chloroplast Isolation Kit

... more info
Minute™ Chloroplast Isolation Kit
Catalog #: CP-011
Price: $198 (50 preps)

Weight: 0.5lbs

Date Added: April 28, 2012
Invent Biotechnologies Minute™ MinuteTM chloroplast isolation kit is composed of optimized chloroplast isolation buffers and filter cartridges with 2.0 ml collection tubes. ... more info
Minute™ Single Cell Isolation Kit

... more info
Minute™ Single Cell Isolation Kit
Catalog #: SC-012
Price: $198 (50 preps)

Weight: 0.5lbs

Date Added: April 28, 2012
Invent Biotechnologies Minute™ single cell isolation kit is composed of optimized tissue disaggregation buffers and specially designed filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate single cells/nuclei from fresh/frozen/formaldehyde fixed animal tissues. ... more info
Minute™  Nuclear Envelope Protein Extraction Kit

... more info
Minute™ Nuclear Envelope Protein Extraction Kit
Catalog #: NE-013
Price: $398 (50 preps)

Weight: 0.5lbs

Date Added: October 2, 2013
The nuclear envelope is a very complex membrane-protein system that is notoriously difficult to isolate and purify due to its connection to nuclear and cytoplasmic components. Traditional method of nuclear envelope isolation and purification requires a large amount of starting material and a lengthy and tedious procedure. Invent Biotechnologies MinuteTM nuclear envelope protein extraction kit is the first commercial kit designed to rapidly isolate nuclear envelope and its associated proteins in native form without using density-gradient and ultra centrifugation. Due to the use of protein extraction filter cartridges, the nuclear envelope protein isolation is simple, easy and user friendly. The nuclear envelope proteins are significantly enriched in the final prep. Unlike traditional method that requires large amount of starting cells/tissues this kit starts with only 10-20 million cells and the buffers are detergent and EDTA free. The procedure can be completed in less than 45 min with a final yield of 10-50 µg protein/sample. ... more info
Minute™ Histone/DNA Binding Protein Extraction Kit

... more info
Minute™ Histone/DNA Binding Protein Extraction Kit
Catalog #: HP-014
Price: $298 (50 preps)

Weight: 0.5lbs

Date Added: April 28, 2012
Histones and other DNA binding proteins play an important role in chromosome organization, gene regulation and posttranslational modification. Core components of histones can be covalently modified through methylation, acetylation, phosphorylation, and sumoylation. These modifications are essential for gene expression, regulation, DNA repair and chromosome condensation. HP-014 is designed to rapidly extract histones and other DNA binding proteins from cultured cells and cells isolated from animal tissues. Unlike many other commercial histone extraction kits that employ acid or high salt extraction methods, HP-014 extracts histone proteins through a completely different mechanism. This kit is equally efficient in extracting histones and other DNA binding proteins that can be used for internal controls for histone modification analysis. In comparison acid extraction protocol selectively extracts basic proteins and a good internal control is usually not possible. HP-014 can extract histones from 0.5 to 5 million cells in less than 10 min with high protein yield (1-2.5 mg/ml) making it the fastest and most robust histone extraction kit available in the market. ... more info
Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls

... more info
Minute™ Total Protein Extraction Kit for Microbes with Thick Cell Walls
Catalog #: YT-015
Price: $98 (50 preps)

Date Added: June 03, 2014
Protein extraction from cell wall-containing microbes is a frequent procedure in bio-research Labs. The methods for protein extraction from these microbes are usually harsh, tedious and not sufficiently reliable. YT-015 provides an instrument-free, rapid and gentle way for extracting proteins from microbes with thick and strong cell walls. These microbes include but not limited to yeast, filamentous fungus, gram positive and negative bacteria, insect eggs and microalgae. This kit contains optimized denaturing and native protein extraction buffers for user to choose from. Unlike many other methods of which harsh conditions, such as 8 M urea, glass bead lysis using an instrument, and boiling the sample with alkaline extraction etc., are used for yeast protein extraction. Proteins in certain molecular weight range are usually preferentially extracted with these methods. This kit features a single tube protocol, full range of yeast proteins are extracted without bias. The kit works well for both log phase and stationary phase microbes. The whole procedure takes less than 10 min to complete and the protein yield is in the range of 2-5 mg/ml. The materials provided are sufficient for 50 extractions. ... more info
Minute™ Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls

... more info
Minute™ Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls
Catalog #: YD-016
Price: $98 (50 preps)

Date Added: June 03, 2014
Protein extraction from cell wall-containing microbes is a frequent procedure in bio-research Labs. The methods for protein extraction from these microbes are usually harsh, tedious and not sufficiently reliable. YD-016 provides a detergent-free, rapid and gentle way for extracting proteins from microbes with thick and strong cell walls. These microbes include but not limited to yeast, filamentous fungus, gram positive and negative bacteria, insect eggs and microalgae. This kit contains optimized detergent-free and EDTA-free protein extraction buffers. The whole procedure takes less than 10 min to complete and the protein yield is in the range of 2-4 mg/ml with un-biased protein representation. This kit features a single tube protocol and works well for both log phase and stationary phase microbes. The materials provided are sufficient for 50 extractions. ... more info
Minute™ Yeast Mitochondria Enrichment Kit

... more info
Minute™ Yeast Mitochondria Enrichment Kit
Catalog #: YM-017
Price: $198 (50 preps)

Date Added: June 03, 2014
Traditional protocols for yeast mitochondria isolation/extraction involve a range of centrifugation-based subcellular fractionation procedures. Typically the techniques include spheroplast preparation, glass-bead lysis using a homogenization instrument, differential centrifugation and several density gradient procedures using a variety of gradient media with ultracentrifugation. The procedures are very tedious and time consuming. Here we feature a simple and rapid protocol for yeast mitochondria enrichment. The procedure is gentle and instrument-free. Nativ mitochondrial proteins can be isolated from yeast in about one hour without ultracentrifugation. This kit contains optimized detergent-free protein extraction buffers. The protein yield is in the range of 150-250 µg/sample. The materials provided are sufficient for 50 extractions. ... more info
Minute™ Plant Microsomal Membrane Extraction Kit

... more info
Minute™ Plant Microsomal Membrane Extraction Kit
Catalog #: MM-018
Price: $398 (50 preps)

Date Added: June 03, 2014
Isolation of microsomal membranes from plant tissues is a common laboratory procedure. Microsomal fraction of plant cell lysate is the focus of interest in many plant research projects. Microsomal fraction is believed to be enriched for plasma membranes, endoplasmic reticulum, Golgi apparatus, vacuolar membranes and other components of membrane system. Traditional method for microsomal fraction isolation involves so called differential pelleting protocol where a series of centrifugation steps are required to obtain various membrane fractions. Traditional protocol for microsomal fraction isolation requires large amount of starting material and employs tedious ultracentrifugation steps. MM-018 offers a simple, rapid and user friendly approach for microsomal membrane extraction using small amount of starting material (200 mg). Water soluble cytosolic proteins are removed during the procedures and water insoluble microsomal fraction, especially plasma membrane fraction, is extracted with optimized buffers in a table-top microcentrfuge. The procedure is simple, rapid and no special instrument required. Native microsomal proteins can be isolated from plant tissue in about one hour without ultracentrifugation. The protein yield is in the range of 100-200 µg/sample. ... more info
MinuteTM Protein/Nucleic Acid Extraction Kit from Gels

... more info
MinuteTM Protein/Nucleic Acid Extraction Kit from Gels
Catalog #: PN-019
Price: $98 (20 preps)

Date Added: June 03, 2014
Passive elution and electro elution are the most common methods for extracting proteins and/or nucleic acid from polyacrylamide/agarose gels. Protein extraction by passive elution usually takes overnight incubation. Eluted proteins are significantly diluted and require further concentration. Protein extraction by electro elution is faster (30 min to 2 h) than passive elution but it requires a special elution device that works less effectively for larger proteins (>70 Kda). The electro buffer usually contains detergent and other chemicals at a concentration that may interfere with downstream applications. Here we feature a rapid and instrument-free protein/nucleic acid extraction kit. Proteins/nucleic acid can be extracted from gels in <10 min with high yield. The elution buffer can be a detergent-containing buffer or pure water depending upon methods of gel staining. The elution volume is between 10 to 200 µl. Multiple gel pieces can be processed in a single tube and the final protein concentration is relatively high. ... more info
Minute™ PCR Template Prep

... more info
Minute™ PCR Template Prep
Catalog #: PCR-020
Price: $98 (100 preps)

Date Added: June 03, 2014
Invent Biotechnologies PCR template Prep kit is designed to extract genomic DNA from animal tissues (such as mouse tail), cultured cells and blood (fresh or anti-coagulated with EDTA, citrate, or heparin) for polymerase chain reaction (PCR). ... more info
Minute™ Protein Extraction Kit for Hair and Nails

... more info
Minute™ Protein Extraction Kit for Hair and Nails
Catalog #: HD-021
Price: $198 (20 preps)

Date Added: November 16, 2014
Proteins account for about 80% of keratinized animal tissues that include but not limited to hair, nail, horn and wool. These tissues contain mainly hard α-keratins made of several distinctive types of protein with molecular weight ranging from 10-135 Kda. Majority of the proteins are ranging from 10-65 Kda. Several methods have been reported for protein extraction from keratinized animal tissues. The extraction buffer usually contains high concentration of urea, thiourea, guanidine hydrochloride or a combination of these strong denaturants. Protein extraction with high concentration denaturants is relatively effective but the concentration of extracted protein is usually low due to larger extraction buffer/tissue ratio. Extracted proteins need to be concentrated prior to further analysis. The presence of high concentration of denaturants may also interfere with downstream application. HD-021 is designed to effectively extract proteins from keratinized animal tissues without using strong denaturants mentioned above. The extracted protein can be directly loaded onto SDS-PAGE without concentration. The extraction buffer of this kit contains 0.5% SDS and other chemicals. Extracted protein concentration is 1-2 mg/ml. ... more info
Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes

... more info
Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes
Catalog #: AT-022
Price: $198 (20 preps)

Date Added: November 16, 2014
Adipose tissue especially white adipose tissue (WAT) has been recognized as an important endocrine and inflammation organ in addition to its energy storage function. Isolation and analysis of proteins from adipose tissues are increasingly critical for understanding many physiological/pathological conditions. However isolation and analysis of WAT and brown adipose tissue (BAT) are technically very challenging due to their high lipid and low protein contents. The water-oil emulsion present in biological sample is notoriously difficult to separate. Our company has developed a novel technology to deal with this issue. A porous filter with unique surface property and pre-defined pore size and thickness coupling with a specially formulated detergent-free extraction buffer is employed to rapidly and effective separate water-oil emulsion derived from adipose tissue homogenate. The extraction buffer has lower freezing point than that of oil in adipose tissues and the aqueous phase can be quickly separated from oil phase by passing the tissue homogenate through the filter. The total proteins isolated are un-biased representation of cellular proteins in the tissue. The extracted proteins concentration is very high (2-3 mg/ml) as compared to other methods. ... more info

							Minute™ Adipose Tissue Fractionation Kit

... more info
Minute™ Adipose Tissue Fractionation Kit
Catalog #: AF-023
Price: $298 (20 preps)

Date Added: November 16, 2014
In average, total cellular proteins account for less than 2% of adipose tissues. Fractionation of adipose tissue is technically very challenging due to its high lipid and low protein contents. Adipose tissue especially white adipose tissue (WAT) has been recognized as an important endocrine and inflammation organ in addition to its energy storage function. Fractionation and analysis of proteins from adipose tissues are critical for understanding many physiological/pathological conditions. The water-oil emulsion present in biological sample is notoriously difficult to separate. We have developed a novel technology to deal with this issue. A porous filter with unique surface property and pre-defined pore size coupling with a specially formulated detergent-free fractionation buffer is the key to fractionate adipose tissue into two fractions: water-soluble protein fraction containing mainly cytosolic proteins and water insoluble fraction containing mainly plasma membrane, and organelles such as mitochondria. The buffers used in this kit are free of primary amine, detergent and reducing agents. Isolated proteins are compatible with all downstream applications including TMT labeling, enzyme digestion, MS analysis and other applications. ... more info

							Minute™ detergent-free nuclei isolation kit

... more info
Minute™ detergent-free nuclei isolation kit
Catalog #: NI-024
Price: $198 (20 preps)

Date Added: September 15, 2015
Invent Biotechnologies MinuteTM> detergent-free nuclei isolation kit is designed to rapidly isolate intact nuclei from animal cultured cells or tissues (fresh or frozen). Unlike many other methods in which detergents are used to lyse the cell membranes, intact nuclei can be isolated from the samples using this patented technology in less than 20 min without using any detergent and a tissue homogenizer. One major problem associates with the use of detergent is the tendency of nuclei aggregation, which is difficult to be separated into single nucleus. To overcome this shortcoming we have developed this detergent-free nuclei isolation kit. The sample is first incubated with buffer A that sensitizes the cells for mechanical disruption. The cell suspension is rapidly passing through a specially designed tilter cartridge. The cell membranes are ruptured when passing through the filter leaving intact native nuclei in the flow through. The nuclei are separated from other small cell debris by low speed centrifugation using a proprietary buffer B. ... more info

							Minute™ Protein Extraction Kit for Fixed and Embedded Tissues

... more info
Minute™ Protein Extraction Kit for Fixed and Embedded Tissues
Catalog #: FE-025
Price: $198 (20 preps)

Date Added: September 15, 2015
Formalin-fixed and paraffin-embedded (FFPE) tissues are widely available and serve as a potential rich source for biomedical research. However protein extraction from FFPE tissue is very challenging due to formalin mediated molecular cross-linking. Traditional methods for protein extraction from FFPE tissues involve repeated deparaffinization and rehydration using organic solvents followed by protein extraction at high temperature with or without optional sonication step. These methods, though relatively effective in some cases, are tedious and time consuming (3-4 h). FE-025 provides a simple and rapid way to extract protein from FFPE tissue without organic solvent deparaffinization. The whole procedure can be completed in less than one hour with a protein yield of 2-3mg/ml. ... more info

Copyright © 2017 Invent Biotechnologies, Inc.. Powered by Zen Cart