Minute™ Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues


Preparation of cytosolic and nuclear fractions from cultured cells and tissues is a common practice. Cell fractionation from cultured cells is relatively easy and straight forward. However, a clear separation of cytosolic and nuclear fractions from tissues, especially frozen tissues, is challenging. The cross-contaminations from frozen tissues are always troublesome due to the altered tissue cellular structure caused by freeze and thaw cycle, improper homogenization and inadequate extraction buffer used. Majority of current commercial kits are designed to treat cultured cells and tissues similarly without paying sufficient attention to the structure difference between the two types of samples. As a result, cross-contamination of cytosolic and nuclear fractions from fresh/ frozen tissues remains as a major problem (see references 1 and 2 below). This novel cytosolic and nuclear extraction kit is specially designed to address the issue by employing proprietary buffers and unique protocol to minimize the cross-contaminations for fresh and frozen tissue samples.

Cat# NT-032   Price:$238/kit (20 preps)  




Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues


Kit Components

Item Amount/Number
Buffer A 25 ml
Buffer B 10 ml
Buffer D 1.5 ml
Buffer N 1.5 ml
Pestle for 1.5 ml tube 2
Protein Extraction Powder 2g